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Dualex叶绿素仪在番茄生理反应的原位估计中的应用

发布时间:2022-05-07 点击量:90

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Dualex便携式智能气象-叶绿素-花青素-黄酮醇测量仪

摘要 

本研究于2020年进行,旨在研究褪黑素(10、50和100µM)在三种方法(浸泡、灌溉和叶面喷洒)下对暴露于根结线虫的番茄幼苗的某些生理方面的影响。幼苗接种了1000株南方根结线虫的感染性幼体[(Kofoid and White)Chitwood]。Dualex®光学传感器用于原位测量总叶绿素、黄酮醇和花青素含量以及氮平衡指数(NBI)。结果表明,虽然褪黑素对叶绿素含量没有显著影响,但褪黑素改善了南方根结线虫对叶绿素的不利影响,这取决于浓度和方式。黄酮醇在灌溉植物中最高,在浸水植物中介于06-1.1之间,表现出显著差异。应用褪黑素的方法会影响NBI,浸泡会促进NBI,而灌溉会导致显著降低。与其他两种方法相比,灌溉可显著提高花青素含量。一个观察结果是,在含有线虫的土壤中施用中低浓度的褪黑素会增加花青素。褪黑素在对抗线虫的反应中具有价值,但需要进一步阐明。

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EFFECTS OF MELATONIN ON ROOT KNOT NEMATODE: IN SITU ESTIMAT ON OF PHYSIOLOGICAL RESPONSES IN TOMATO

Abstract

This research was conducted in 2020 to investigate the influence of melatonin (10, 50 and 100 µM) given in three methods (immersion, irrigation and foliar spraying), on some physiological aspects of tomato seedlings exposed to root knot nematodes. The seedlings were inoculated with 1000 infective juveniles of Meloidogyne incognita [(Kofoid and White) Chitwood]. Dualex ® optic sensor was used to in situ measure total chlorophylls, flavonols and anthocyanins contents and nitrogen balance index (NBI). Results indicated that while no significant effects were observed on chlorophyll content, melatonin ameliorated the adverse effects of M. incognita on chlorophyll depending on the concentration and mode. Flavonols were at the highest in the irrigated plants and the lowest in the immersed ones, between 06-1.1, exhibiting a significant difference. NBI was affected by the method the melatonin was applied, and immersing boosted it, while irrigation caused a significant decrease. Irrigation resulted in significantly higher anthocyanins compared to the other two methods. One observation was that applying melatonin in the low and medium concentrations to the nematode containing soil increased the anthocyanins. Melatonin merits a value in developing a response against the nematode but needs further elucidation.

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